Comparison of different sources of platelet-rich plasma as treatment option for infertility-causing endometrial pathologies

ObjectiveTo study the effect of human plasma from different sources, namely, umbilical cord blood and adult platelet-rich (PRP), on regeneration endometrial damage.DesignComposition analysis, in vitro approaches, a preclinical murine model using to promote regeneration.SettingHospital university laboratories.Patient(s)/Animal(s)Adult four Asherman syndrome/endometrial atrophy patients one fertile woman, commercial plasma, uterine-damaged NOD/SCID mice were used.Intervention(s)Endometrial stromal cells primary culture an stem cell line cultured vitro, treated with samples several origins.Main Outcome Measure(s)To investigate possible beneficial effects PRP patients. To test if had stronger than regeneration. demonstrate was as effective healthy woman could therefore be used for autologous treatment.Result(s)All contained molecules high potential (stem factor, platelet-derived growth factor BB, thrombospondin-1, von Willebrand factor). Furthermore, highest increase proliferation migration rate found when plasma; also revealed significant increment. In mouse model, higher expression Ki67 Hoxa10 endometrium detected after applying PRP, proteomic analysis specific protein profile depending treatment. The damaged uterine tissue showed more proregenerative markers (Stat5a, Uba3, Thy1) compared other treatments (nonactivated activated no treatment).Conclusion(s)Human possesses properties usable pathologies. Besides that, these regenerative seem apparent source obtaining is blood. damage. Composition Hospital laboratories. Adult used. Endometrial origins. All treatment). Human